Jump to content Jump to navigation

Morphological facilities

A wide range of microscopy techniques and other morphologic analyses.

Jeol JEM1400plus TEM
Jeol JSM7600F FEG SEM
Zeiss Xradia 520 Versa micro-CT
Skyscan 1172 micro-CT
TEM fixation and staining lab
TEM ultra-microtomy lab
LM fixation and staining lab
LM microtomy lab
DIC microscopy lab & Stacking microscopy lab
Dissecting microscopy lab
Flow cytometer and Cavitron

Jeol JEM1400plus TEM

Generates very detailed images at very high magnifications of biological and geological materials. To study ultra-structures on a cellular level. For example wall sections of pollen grains, composition of wood vessels.

 

 Back to top

Jeol JSM7600F FEG SEM

Generates very detailed images at very high magnifications of biological and geological material. Is also equipped with EDS (Energy-dispersive X-ray spectroscopy) to analyze chemical elements.

Back to top

Zeiss Xradia 520 Versa micro-CT

Tomography: 3D imaging by sections or sectioning through the reconstruction 2D X-ray images. Operators can select different focal planes which contain the structures of interest.

With this non-destructive method the internal structure of an object can be studied down to a scale of 0,3 microns. For this purpose, a series of X-ray images is taken of the rotating object after which the projections obtained are used to create a three-dimensional model of its internal structure.

Back to top

Skyscan 1172 micro-CT

Tomography: 3D imaging by sections or sectioning through the reconstruction 2D X-ray images. Operators can select different focal planes which contain the structures of interest.

With this non-destructive method the internal structure of an object can be studied down to a scale of 30 microns. For this purpose, a series of X-ray images is taken of the rotating object after which the projections obtained are used to create a three-dimensional model of its internal structure.

Back to top

TEM fixation and staining lab

In this laboratory, samples are fixed, embedded and stained for Transmission Electronic Microscopy.

1. Oven. For polymerizing of EPON (epoxy resin) which is used to embed samples for ultra-thin sectioning.
2. PH-meter. To adjust the acidity of fixing agents and culture media.
3. Ultrasonic bath. Can be used to clean samples and small objects. Is also used to accelerate dissolution.
4. Knife maker. To accurately break glass strips to make glass knives.

Back to top

TEM ultra-microtomy lab

In this laboratory, samples are sectioned for analyses with the Transmission Electron Microscope.

1. Pyramitome. For trimming of EPON blocks to get a small surface containing the material that needs to be sectioned ultra-thin. The trimming is done with glass knives.
2. Heat plate. During the trimming process, EPON sections are stained on this plate to enable finding the position of the material in the EPON block.
3. Rotation microtome with conveyor belt. Cutting machine for paraffin material. The paraffin sections are deposited as a ribbon on the conveyer belt.

4. Ultra microtome. Cutting machine for ultra-thin sections. This is enclosed by two screens to prevent air movement. The sections are made with diamond knives.

Back to top

LM fixation and staining lab

In this laboratory, microscopic slides are prepared for fine anatomical surveys.

1. Ovens. To dry and cure microscopic slides and for impregnating with paraffin or to polymerize resin blocks.
2. Paraffin embedding station. This appliance helps to heat paraffin and to cast paraffin blocks.

3. Vacuum pump. Used to pump air from samples so that they can be well impregnated.
4. Precision scales. Used for weighing out chemicals.
5. Critical point dryer. Special technique to dry material without changes in shape, suitable for scanning electron microscopy. The gas cylinder next to the machine contains carbon dioxide which is used in this process.
6. Water bath. For accurately heating samples. For example to macerate woody tissue so that the separate vessel elements and fibers can be observed.
7. Heated desiccator. This is a heated container that can be attached to a vacuum pump to remove air from samples in molten paraffin.

Back to top

LM microtomy lab

In this laboratory, samples are sectioned for further analyses with the Light Microscope.

1. Motorized microtome. To cut sections from material embedded in plastic resin. The motor ensures smooth and uniformly thin sections. Section thickness 1.5 – 7 micrometer.
2. Rotation microtome. To cut sections from material embedded in plastic resin. Section thickness 3 – 7 micrometer.
3. Rotation microtome. To cut sections from material embedded in paraffin. Section thickness 5 – 15 micrometer.
4. Sliding microtome. For sectioning wood samples without embedding. Section thickness 10 – 30 micrometer.
5. Freeze-Sliding microtome. To enable sectioning of porous material that can be mounted by freezing. Section thickness 10 – 30 micrometer.

Back to top

DIC microscopy lab & Stacking microscopy lab

In the DIC microscopy laboratory, researchers and students can photograph their study material using various microscopy techniques. In the Stacking microscopy laboratory, researchers and students can photograph their study material using stacking microscopy.

1. Carl Zeiss AxioImager M2 compound microscope with differential interference contrast (DIC) and stacking photography. The different lighting options make it possible to highlight 3-dimensional, translucent structures in microscopic slides. Magnifications from 50x – 630x and 1000x (immersion oil).
2. Carl Zeiss Discovery V20 stereo microscope for stacking photography. By merging several photographs of one sample, at different focal planes, a single final photograph is created in which the entire sample is in focus. For the photography and study of (un)dissected material with magnifications from 7.5x – 150x.

3. Carl Zeiss Discovery V20 stereo microscope for stacking photography. For the photography and study of (un)dissected material with magnifications from 7.5x – 150x.
4. Carl Zeiss Discovery V8 stereo microscopes (5x reflected light, 1x reflected light / transmitted light) manually operated. For the study of (un)dissected material with magnifications from 10x – 80x.

Back to top

Dissecting microscopy lab

In this laboratory, researchers and students can study their material using conventional microscopy.

4. Carl Zeiss Discovery V8 stereo microscopes (5x reflected light, 1x reflected light / transmitted light) manually operated. For the study of (un)dissected material with magnifications from 10x – 80x.

Back to top

Flow cytometer and Cavitron

In this laboratory, genome size, ploidy level (the number of sets of chromosomes in the nucleus of a cell) and sap transport of freshly collected samples are measured.

1. Flow cytometer. Can show the amount of DNA in the nucleus of a cell. This information can assist to ascertain the species of a plant and to determine if a plant is polyploid (has multiple copies of one or more chromosomes).
2. Ultra centrifuge. This centrifuge has a special rotor that can be used to determine the speed of sap transport in pieces of plant stem.

Back to top



For more information about our Morphological facilities, please contact Arjen Speksnijder, Head of Laboratories

arjen.speksnijder@naturalis.nl