Protocols (EN)
Many different chemicals are used for collecting specimen for Natural History Collections. Some of these may have negative effects on DNA. A short section in the collection protocol is devoted to this, but we have also gathered all our knowledge in these extensive guidelines. If any chemical that you use is still missing, please contact us.
Extensive guidelines for preserving specimen or tissue for later DNA work
Version 1.0 by Camiel Doorenweerd & Kevin Beentjes, Jan 2012
At the barcoding facility we use a Thermo Fisher KingFisher Flex for the DNA extractions, with slightly modified protocols from those of the manufacturers kits. We modified all steps to be on the safe side, as we commonly work with low concentrations of DNA.
To save the robot protocols use right click > save as...
Macherey Nagel (NucleoMag)
Qiagen (BioSprint)
Thermo Fisher (KingFisher DNA Kit)
Friday, February 3, 2012