DNA Barcoding
Projects
Facilities & Methods
Protocollen (Dutch)
Protocols (English)
Ambition
In 2010 NCB Naturalis started a DNA barcoding program, aiming to produce 12 000 DNA barcodes annually. A single DNA barcode is defined as the section of the genome that is internationally accepted (IBOL, ECBOL) to be used for species recognition. For a general introduction on DNA barcoding, see the Barcode of Life website.
To enable high-throughput we work with 96-wells plates, of which one position is reserved for the negative control. This means that we require specimen input in multiples of 95. To get an indication of the variation of the DNA barcodes within a species and between species, a rule-of-thumb is that three specimens per species can be barcoded. Because of potential failure, some more specimens per species can be collected.
Flora and Fauna of the Netherlands
The Flora and Fauna of the Netherlands will receive special attention. The goal is to cover the entire flora and fauna of the Netherlands. For this we are after freshly collected material, identified by specialists in multiples of 95. If you fit this image and see the value of the barcodes for your research, please do not hesitate to contact us. All specimens collected thus far are registered online at Soortenregister.nl - search for a species and select the 'DNA barcode' tab. At the projects section on this website you will find direct links to the lists of collected specimen for each project at the soortenregister website.
LtRTtB: Frank Stokvis, Kevin Beentjes, Martijn de Jong, Els Baalbergen, Roland Butôt, Oscar Vorst, Aline Nieman
Thursday, January 10, 2013